Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding approaches for tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds: (A) Wi38 monoculture, (B) Calu-3 monoculture, and (C) coculture.

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques:

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding of the OL of tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds using Wi38 cells. (A) Cell metabolic activity of Wi38 cells on the OL and IL on days 1, 5, and 7. (B) DNA quantification on the OL and IL at day 7. (C) Coverage of the IL by Wi38 cells at day 7. Representative images of Wi38 cells growing on the OL using three seeding densities: (D) 1.5 × 10 5 cells/cm 2 , (E) 3 × 10 5 cells/cm 2 and (F) 6 × 10 5 cells/cm 2 . Cells were treated with DAPI (blue) and phalloidin (red) to stain the nuclei and actin filaments, respectively. Images were captured via confocal microscopy (Axio Examiner.Z1 confocal microscope). Results displayed as mean ± SEM ( n = 3, * p < 0.05, ** p < 0.01).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Staining, Confocal Microscopy, Microscopy

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic acid (t-PCL-CHyA-B) scaffolds seeded on the outer layer (OL) with Wi38 cells and the inner layer (IL) with Calu-3 cells for 4h under rotation using 3D printed accessories. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds seeded with (C) Wi38 monoculture, (D) Calu-3 monoculture and (E) coculture using 3D printed accessories. Images were obtained using a Axio Examiner. Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3, ** p < 0.01, **** p < 0.0001).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Microscopy, Staining

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds seeding the OL with Wi38 cells and the IL with Calu-3 cells for 2 h under rotation. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds: (D) Wi38 monoculture, (E) Calu-3 monoculture, and (F) coculture. Images were obtained using an Axio Examiner Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Microscopy, Staining